Non-biological gene carriers designed for overcoming the major extra- and intracellular hurdles in gene delivery, an updated review
نویسندگان
چکیده مقاله:
Gene therapy as a modern therapeutic approach has not yet advanced to a globally-approved therapeutic approach. Lack of adequate reliable gene delivery system seems to be one of the major reasons from the pharmaceutical biotechnology point of view. Main obstacles delaying successful application of human gene therapy are presented in this review. The unique advantages of non-biological gene carriers as compared to their biological counterparts make them ideal alternatives for overcoming extra- and intracellular barriers in a more safely manner. We, therefore, highlight the significant contributions in non-biological gene delivery and favorable characteristics of different design attitudes with focus on in vivo approaches. Bypassing the rapid extracellular enzymatic degradation of genetic materials is covered in extracellular segment of this review with emphasis on PEGylated and targeted formulations. The successful approaches to pave the rest of the way from cellular uptake to intracellular transfer and gene expression of unpacked DNA are also discussed. From these approaches, we emphasize more on optimization of cationic-based polymers and dendrimers, developing newly designed membrane-effective components, and adjusting the hydrophilic-hydrophobic balance of the synthesized vectors
منابع مشابه
non-biological gene carriers designed for overcoming the major extra- and intracellular hurdles in gene delivery, an updated review
gene therapy as a modern therapeutic approach has not yet advanced to a globally-approved therapeutic approach. lack of adequate reliable gene delivery system seems to be one of the major reasons from the pharmaceutical biotechnology point of view. main obstacles delaying successful application of human gene therapy are presented in this review. the unique advantages of non-biological gene carr...
متن کامل“ Gene Therapy : An Updated Review ”
Novel treatment modalities like gene therapy and cell therapy are attractive for such diseases where current therapies have failed. Although the clinical potential of these therapies has been demonstrated in the West, their use still remains in experimental stages. In India a number of groups are carrying out basic research on gene delivery vector development and strategies for cancer gene ther...
متن کاملEffects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers
BACKGROUND Efforts to improve the efficiency of non-viral gene delivery require a better understanding of delivery kinetics of DNA molecules into clinically relevant cells. Towards this goal, three DNA molecules were employed to investigate the effects of DNA properties on cellular delivery: a circular plasmid DNA (c-DNA), a linearized plasmid DNA (l-DNA) formulated by single-site digestion of ...
متن کاملRationally Designed Synthetic Vectors for Gene Delivery
Vector development is one of the most important challenges facing the successful use of genes for treatment of diseases. Although chemically produced vectors offer distinct advantages over biological systems such as viruses, there are still some hurdles that have to be overcome before synthetic gene delivery vectors can be successfully implemented. This brief review discusses the biological bar...
متن کاملNucleic acid carriers for pulmonary gene delivery
The delivery of nucleic acids to the pulmonary route appears to be promising due to non-invasiveness, large area of epithelial surface lining the lung, easy accessibility and ability to provide a platform for local delivery. The therapeutic role of nucleic acids in various diseases pertaining to the pulmonary route e.g. cystic fibrosis, α1-antitrypsin deficiency, asthma, chronic obstructive pul...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 2 شماره 1
صفحات 1- 20
تاریخ انتشار 2015-01-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023